Huazhong Agricultural University, College of Plant Science and Technology. Shizishan 1, Hongshan district,Wuhan City,Hubei Province,China 430070

Tel: (27)-87281242 • E-mail:cxluo@mail.hzau.edu.cn or  Luo.chaoxi@gmail.com

 

SUMMARY

Accomplished Plant pathology professional with extensive background in Molecular biology, Microbiology, Genetics, and Pest (especially fungi) Management.

EDUCATION

Ph.D. in Molecular Plant Pathology

Kagoshima University, Kagoshima, Japan, 2003.4-2006.3

Dissertation: Genetic Analysis and Chromosomal Assignment of Cultivar-Specific Avirulence Genes in Rice Blast Fungus

Master of Agriculture in Plant Pathology

Saga University, Saga, Japan, 2001.4-2003.3

Thesis: Genetic Analysis of Avirulence Genes in Rice Blast Fungus Magnaporthe oryzae

Bachelor of Agriculture in Plant Pathology

China Agriculture University, Beijing, China, 1991.9-1995.7

PROFESSIONAL EXPERIENCE

Professor: 2008.8-present, College of Plant Science and Technology & Key Lab of Crop Disease Monitoring & Safety Control in Hubei Province, Huazhong Agricultural University, Wuhan 430070, China.

• Molecular mechanisms of fungicide (DMIs, QoIs and SDHIs) resistance in peach brown rot fungi Monilinia spp.

• Molecular mechanisms of resistance of rice blast fungus Magnaporthe oryzae to fungicide Isoprothiolane and rice false smut fungus Ustilaginoidea virens to DMI and QoI fungicides.

• Identification of resistance of rice cultivars to rice false smut disease and integrated management of rice false smut fungus.

Postdoctoral Research Associate: 2006.4-2008.7, the Department of Entomology, Soils and Plant Sciences, Clemson University, Clemson, SC 29634-0315, USA. Research Advisor: Dr. Guido Schnabel: (864) 656-6705, schnabe@clemson.edu

• Resistance risk assessment of brown rot fungus Monilinia fructicola to quinone outside inhibitor (QoI), demethylation inhibitor (DMI), and benzimidazole (BZI) fungicides.

• Conduction of expression analysis of the cytochrome P450 lanosterol 14α-demethylase gene (MfCYP51) and ABC transporter gene (MfABC1) in M. fructicola isolates.

• Sequencing MfCYP51 gene and upstream region to identify mechanism causing DMI fungicide resistance in M. fructicola.

• Cloning and sequence analysis of cytochrome b gene (Cyt b) to identify mechanism causing QoI fungicide resistance in M. fructicola.

• Development of molecular based methods to rapidly detect DMI and/or QoI fungicides resistant isolates.

Ph.D. Graduate Research Assistant: 2003.4-2006.3, the United Graduate School of Agricultural Sciences, Kagoshima University, Kagoshima 890-8580, Japan. Research Advisors: Dr. Kusaba Motoaki: (81) 952-288727, mkusaba@cc.saga-u.ac.jp and Dr. Yaegashi Hiroshi: (81)197-651597, yae3h@amber.plala.or.jp

• Mapped three avirulence genes of M. oryzae by using RAPD, RFLP and telomeric markers.

• Chromosomal assignment of three avirulence genes AvrPik, AvrPiz and AvrPiz-t on the 1.6-Mb chromosome, chromosome 3 and chromosome 7 of M. oryzae, respectively, by using CHEF electrophoresis combined with Southern hybridization.

• Characterized the significance of mini-chromosome in M. oryzae.

M.S. Graduate Research Assistant: 2001.4-2003.3, Faculty of Agriculture, Saga University, Saga, 840-8502, Japan. Research Advisor: Dr. Yaegashi Hiroshi: (81)197-651597, yae3h@amber.plala.or.jp

• Genetically analyzed the relationships of three pairs of avirulence genes in the same gene families, respectively.

• Identified three avirulence genes AvrPik, AvrPiz and AvrPiz-t in rice-field isolates.

Research Assistant: 1995.7-2000.6, Plant Protection Research Institute, Yunnan Academy of Agricultural Sciences, Kunming, Yunnan, China. Research Advisor: Prof. Chengyun Li

• Sampling rice blast fungus Magnaporthe oryzae from 6 different counties in Yunnan province.

• Single-spore isolation and maintenance of M. oryzae isolates.

• Genetic analysis of avirulence genes in M. oryzae.

Research Associate: 2000.7-2000.9, Plant Protection Research Institute, Yunnan Academy of Agricultural Sciences, Kunming, Yunnan, China. Research Advisor: Prof. Chengyun Li

• Identification of avirulence genes in rice blast fungus M. oryzae.

 

CURRENT AND PENDING PUBLICATIONS

I. English Publications

1. Yin, L. F., Hu, M. J., Wang, F., Kuang, H., Zhang, Y. Schnabel, G., Li, G. Q. and Luo, C. X. 2012. Frequent gain and loss of introns in fungal cytochrome b genes. PLoS ONE 7(11): e49096. doi:10.1371/journal.pone.0049096  (Corresponding author).

2. Hu, M. J., Cox, K. D., Schnabel, G. and Luo, C. X.  2011. Monilinia species causing brown rot of peach in China. PLoS ONE, 6(9): e24990. Doi:10.1371/journal.pone.0024990 (Corresponding author).

3. Hu, M. J., Yin, L. F., Chen, Y., Chen, S. N., Liu, X. L., Chen F. P., and Luo, C. X. 2011. A group I intron located downstream of the G143 position of the Cyt b gene in Monilinia fructicola is present in genetically diverse populations from China. In Modern Fungicides and Antifungal Compounds VI, pp 143-150, Dehne, H.W., Deising, H.B., Gisi, U., Kuck, K.H., Russell, P.E., Lyr, H. (Eds.), Deutsche Phytomedizinische Gesellschaft, Braunschweig, Germany (Corresponding author).

4. Hu, M. J., Luo, C. X., Grabke, A. and Schnabel, G. 2011. Selection of a suitable medium to determine sensitivity of Monilinia fructicola mycelium to SDHI fungicides. Journal of Phytopathology, 159: 616-620 (Corresponding author).

5. Hu, M. J., Chen, Y., Chen, S. N., Liu, X. L., Yin, L. F.  and Luo, C. X. 2011. First report of brown rot of peach caused by Monilinia fructicola in southeastern China. Plant Disease, 95: 225. (Corresponding author)

6. Grabke, A., Hu, M. J., Luo, C. X., Bryson, P. K. and Schnabel, G. 2011. First report of brown rot of apple caused by Monilinia fructicola in Germany. Plant disease, 95: 772.

7. Yin, L. F., Luo, C. X., Kusaba, M. and Yaegashi, H. 2010. Analysis of the abnormal segregation of pathogenicity in Magnaporthe grisea by using a genetic cross of Oryza and Eleusine isolates. Agricultural Sciences in China, 9:383-391. (Corresponding author)

8. Luo, C. X,, Hu, M.J.,Jin, X., Yin, L. F., Bryson, P. K., and Schnabel, G. 2010. An intron in the cytochrome b gene of Monilinia fructicola mitigates the risk of resistance development to QoI fungicides. Pest Management Sciences, 66:1308-1315.

9. Luo C. X, Cox, K. D., Amiri, A., and Schnabel, G. 2008. Occurrence and detection of the DMI resistance-associated genetic elemen‘Mona’ in Monilinia fructicola. Plant Disease, 92: 1099-1103.

10. Luo C. X, and Schnabel, G. 2008. The cytochrome P450 lanosterol 14α-demethylase gene is a demethylation inhibitor fungicide resistance determinant in Monilinia fructicola field isolates from Georgia.  Applied and Environmental Microbiology, 74: 359-366.

11. Luo C. X, and Schnabel, G. 2008. Adaptation to fungicides in Monilinia fructicola isolates with different fungicide resistance phenotypes. Phytopathology, 98: 230-238.

12. Kusaba, M., Luo C. X., Hanamura, H., Misaka, M., Mochida, T., Fujita, Y., and Tosa, Y. 2008. An avirulence gene to rice cultivar K60 is located on the 1.6-Mb chromosome in Magnaporthe oryzae isolate 84R-62B. Journal of General Plant Pathology, 74: 250-253.

13. Luo, C. X., Yin, L. F., Ohtaka, K., and, Kusaba, M. 2007. The 1.6 Mb chromosome carrying the avirulence gene AvrPik in Magnaporthe oryzae isolate 84R-62B is a chimera containing chromosome 1 sequences. Mycological Research, 111: 232-239.

14. Luo, C. X., Yin, L. F., Koyanagi, S., Farman, M. L., Kusaba, M., and Yaegashi, H. 2005. Genetic mapping and chromosomal assignment of Magnaporthe oryzae avirulence genes AvrPik, AvrPiz and AvrPiz-t, controlling cultivar specificity on rice. Phytopathology, 95: 640-647.

15. Luo, C. X., Yasuda, N., Iwano, M., Tanaka, H., Kusaba, M., and Yaegashi, H. 2005. Identification of Magnaporthe oryzae avirulence gene corresponding to the rice blast resistance gene Pik-m. Bull. Fac. Agr., Saga Univ., 90: 15-21.

16. Luo, C. X., Fujita, Y., Yasuda, N., Hirayae, K., Nakajima, T., Hayashi, N., Kusaba, M., and Yaegashi, H. 2004. Identification of Magnaporthe oryzae avirulence genes to three rice blast resistance genes. Plant Disease, 88: 265-270.

17. Luo, C. X., Hanamura, H., Sezaki, H., Kusaba, M., and Yaegashi, H. 2002. Relationship between avirulence genes of the same family in rice blast fungus Magnaporthe grisea. Journal of General Plant Pathology, 68: 300-306.

18. Kusaba, M., Urata, H., Fukunaga, Y., Luo, C. X., Li, C. Y. and Yaegashi, H. 2001. Catenulate conidia formed by mutants of the rice blast fungus. Southwest China Journal of Agricultural Sciences, 14: 6-12.

19. Li, C. Y., Yang, Q. Z., Zhen, F. P., Luo, C. X., Li, J. B., and Ise, K. 1997. Cross-fertility and pathogenicity of the blast fungus Magnaporthe grisea Sacc. Isolated from cultivated and wild rice in Yunnan. International Rice Research Notes, 22: 14-15.

 

II. Chinese Publications

20. Yin, L. F., Hu, M. J., Jin, X., Schnabel, G., and Luo, C. X. 2010. Progress in molecular mechanisms of fungicide resistance in peach brown rot fungi Monilinia spp. Plant protection, 36:28-32 (Corresponding author).

21. Luo, C. X., Li, J. B., Li, C. Y., Yin, L, F., Zhou, X. G., and Wang, F.  2000. Race evolution of Magnaporthe grisea and evaluation of rice vertical resistant genes in Yunnan. Southwest China Journal of Agricultural Sciences, 13: 57-61.

22. Luo, C. X., Li, J. B., and Li, C. Y. 2000. Genetic study of melanin in Magnaporthe grisea. Southwest China Journal of Agricultural Sciences, 13: 79-82.

23. Luo, C. X., Li, J. B., and Li, C. Y. 1999. Expression of genes controlling perfect state and melanin in Magnaporthe grisea. Acta Phytophylacica Sinica, 26: 107-110.

24. Luo, C. X., Li, J. B., and Li, C. Y. 1998. Genetic Analysis of Sporulation in Pyricularia grisea. Southwest China Journal of Agricultural Sciences, 11: 64-67.

25. Li, C. Y., Luo, C. X., Li, J. B., Shen, Y., and Ise, K. 2000. Mapping avirulence gene in the rice blast fungus Magnaporthe grisea. Scientia Agricultura Sinica, 33: 49-53.

26. Li, C. Y., Luo, C. X., Hayashi, N., and Naito, H. 1997. Segregation for pathogenicity of 3 crosses in rice blast fungus, Magnaporthe grisea. Acta Phytophylacica Sinica, 24: 293-296.

27. Li, C. Y., Shen, Y., Yuan, X. P., Luo, C. X., and Zhao, X. H. 1997. Inheritance of avirulence/virulence in a cross of two isolates of Magnaporthe grisea derived from upland rice plant. Scientia Agricultura Sinica, 30: 30-36.

28. Li, C. Y., Chen, Q. Z., Chen, Z. Q., Luo, C. X., Hayashi, N., and Ise, K. 1996. Distribution of the mating type in Magnaporthe grisea population pathogenic to rice in Yunnan Province. Scientia Agricultura Sinica, 29: 60 - 64.

29. Li, J. B., Luo, C. X., and Li, C. Y. 2001. Race composition and distribution of Magnaporthe grisea Cavara in Yunnan Province in recent years. Plant Protection, 27: 12-14.

30. Li, J. B., Luo, C. X., Li, C. Y. and Yin, L. F. 2000. Analysis and evaluation of a part of rice varieties resistant to Magnaporthe grisea in Yunnan Province. Plant Protection, 26: 1-4.

31. Li, J. B., Li, C. Y., Luo, C. X., Yin, L. F., Zhou, X. G. and Chen Y. 2002. Genetic Analysis of Avirulence Genes in Two Isolates of Rice Blast Fungus, Magnaporthe grisea. Journal of Yunnan Agricultural University, 17: 328-330.

32. Shen, Y., Li, C. Y., Zhao, X. H., Luo, C. X., and Wang, Y. L. 1997. The Phenomenon of hyphal fusion in Magnaporthe grisea and the Pathogenicity of its progeny. Scientia Agricultura Sinica, 30: 16-22.

33. Zhou, X. G., Zhu, L. H., Zhai, W. X., Li, J. B., Luo, C. X., and Li, C. Y.  2002. The construction of bacterial artificial chromosome (BAC) library of rice blast pathogen. Journal of Yunnan Agricultural University, 17: 331-334.

 

RESEARCH PRESENTATIONS

1. Luo, C. X.,  Hu, M. J., Cox, K. D.,  Schnabel, G. 2011. Monilinia species in China- Surprising facts. (Talk presented at The American Phytopathological Society and The International Association for the Plant Protection Sciences (IAPPS) Joint Meeting, Honolulu, Hawaii, USA, August 6-10, 2011).

2. Luo, C. X., Hu, M. J., Yin, L. F., Chen,Y., Chen, S. N., Liu, X. L., Chen F. P. 2010. A group I intron located downstream of the G143 position of the Cyt b gene in Monilinia fructicola is present in genetically diverse populations from China. (Talk presented at the 16th International Reinhardsbrunn Symposium, Friedrichroda, Germany, April 25-29,2010).

3. Luo, C. X., and Schnabel, G. 2007. The MfCYP51 gene is a DMI fungicide resistance determinant in Monilinia fructicola field isolates (Talk presented at the Southeastern Professional Fruit Workers Conference, Byron, GA, USA, October 24-26).

4. Luo, C. X., and Schnabel, G. 2007. Adaptation to fungicides in Monilinia fructicola isolates with different fungicide sensitivity phenotypes. Phytopathology 97:S68 (Talk presented at the American Phytopathological Society annual meeting, San Diego, CA, USA, July 28-August 1, 2007).

5. Luo, C. X., Yin, L. F., Koyanagi, S., Kusaba, M., and Yaegashi, H. 2004. Chromosomal location of avirulence gene AvrPik in rice blast fungus Magnaporthe oryzae, determined by using clamped homogenous electric field (CHEF) electrophoresis. Japanese Journal of Phytopathology 70:192-193 (Talk presented at the annual meeting of the Phytopathological Society of Japan, Fukuoka, March 28-30, 2004).

6. Luo, C. X., Koyanagi, S., Yin, L. F., Kusaba, M., and Yaegashi, H. 2004. Genetic mapping of avirulence genes in the rice blast fungus with telomeric markers. Japanese Journal of Phytopathology 70:29 (Talk presented at the meeting of the Kyushu Division of the Phytopathological Society of Japan, Miyazaki, September 18, 2003).

7. Luo, C. X., Fujita, Y., Yasuda, N., Hayashi, N., Kusaba, M., and Yaegashi, H. 2003. Identification of avirulence genes in rice blast fungus corresponding to resistance genes Pik and Piz. Japanese Journal of Phytopathology 69:25 (Talk presented at the meeting of the Kyushu Division of the Phytopathological Society of Japan, Isahaya, September 19, 2002).

8. Luo, C. X., Kirayae, K., Nakajima, T., Fujita, Y., Yasuda, N., Hayashi, N., Kusaba, M., and Yaegashi, H. 2002. Linkage analysis of avirulence genes and RAPD markers in rice blast fungus. Japanese Journal of Phytopathology 68:172-173 (Talk presented at the annual meeting of the Phytopathological Society of Japan, Osaka, April 3-5, 2002).

9. Schnabel, G. and Luo, C. X. 2007. Mechanism of DMI fungicide resistance in Monilinia fructicola and evidence of predisposition to QoI fungicides. (Talk presented at the 83rd Annual Cumberland-Shenandoah Fruit Workers Conference, Winchester, VA, November 14-16, 2007)

10. Yin, L. F., Imamura, F., Luo, C. X., Kusaba, M., and Yaegashi, H. 2004. Abnormal segregation of retrotransposon Grasshopper observed in F1 progeny isolates of Magnaporthe oryzae. Japanese Journal of Phytopathology 70:29-30 (Talk presented at the meeting of the Kyushu Division of the Phytopathological Society of Japan, Miyazaki, September 18, 2003).

 

TEACHING EXPERIENCE

Teaching Assistant: The United Graduate School of Agricultural Sciences, Kagoshima University, Japan.

• Special Research in Molecular Plant Pathology. For Master graduate students, 2003, 2004, 2005.

• Graduate Research in Plant Pathology. For undergraduate students, 2003, 2004, 2005.

• Experiments in Plant Pathology. For undergraduate students, 2003, 2004, 2005.

Teaching Assistant: The Faculty of Agriculture, Saga University, Japan.

• Graduate Research in Plant Pathology. For undergraduate students, 2002.

• Experiments in Plant Pathology. For undergraduate students, 2002.

Professor: The College of Plant Science and Technology, Huazhong Agricultural University, China.

For undergraduate students

• Regulations on Animal and Plant Quarantine , 2008.

• Plant Pathology, 2009, 2010, 2011.

• Professional Engilish for Plant Protection, 2012,2013.

• Agricultural Plant Pathology, 2013.

For foreign students

• Plant Pathology, 2010, 2011.

For Ph. D students

• Seminar for Plant Pathology, 2013.

AWARDS

• Scientific and Technological progress Award, 2nd prize. 1998. Ministry of Agriculture of China.

• Scientific and Technological progress Award, 2nd prize. 1999.  China

• Scientific and Technological progress Award, 1st prize. 1999. Yunnan province of China

• Excellent  teaching quality Award, 3rd prize. 2009. Huazhong Agricultural University

GRANTS

• “Managing fungicide resistance in Monilinia fructicola; a crucial undertaking to sustain peach production in the Southeast”. November, 2007, written in collaboration with PI Dr. Guido Schnabel.

• “Molecular mechanism of fungicide resistance in plant pathogenic fungi”. 2008-2011, ¥500,000 funded by Huazhong Agricultural University under the project of the Scientific Research Foundation for the Returned Overseas Chinese Scholars (no. 52204- 08019).

• “Molecular mechanism of tricyclazole resistance and establishment of simple resistance detection methods in Magnaporthe oryzae”. 2009-2011, ¥100,000 funded by Ministry of Education (no. 109114).

• “Molecular mechanism of Isoprothiolane resistance in Magnaporthe oryzae”. 2009-2011, ¥500,000 funded by Ministry of Education under the project of New Century Excellent Talented Scholars (no. NCET-08-0783).

• “Management of false smut of rice”. 2009-2013, ¥2,580,000 funded by Ministry of Agriculture under project of the R&D Special Fund for Public Welfare (no. 200903039-3).

• “Molecular mechanism of effect of 1166-bp intron in Cyt b gene to the G143A point muation”. 2011-2013, ¥320,000 funded by Ministry of National Natural and Science Found of China (no. 31071703).

 • “Monitoring and management for fungicide resistance in pathogens of major crops”. 2013-2017, ¥1,630,000 funded by Ministry of Agriculture under project of the R&D Special Fund for Public Welfare (no. 201303023).

 

PROFESSIONALISM

• The American Phytopathological Society (APS), 2006-present.

• The Phytopathological Society of Japan, 2001-2006.

• The Chinese Society for Plant Pathology, 1996-2000,2008-present.

• Hubei Province Society for Plant Pathology, 2008-present.

• Reviewer for international journals <Plant disease>, <Pesticide Biochemistry and Physiology>, <Hortscience>.